Difference between revisions of "Part:BBa K3179102:Design"

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===Design Notes===
 
===Design Notes===
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This part consist with promoter pCMV can basic part rtTA3. pCMV is a strong mammalian expression promoter derived from human cytomegalovirus, usually used for routine expression, with high expression levels but may be inhibited in some cells. rtTA is a key component of Tet-On system, and rtTA3 is third generation rtTA. rtTA3 is an activation factor of pTRE. rtTA3 isn’t active when it is present alone, and it can be active when combine with doxycycline (DOX), then the complex can combine to pTRE and start the downstream transcription. When DOX get removed the rtTA3 will became no activation again. In our experiment, pCMV-rtTA3 is used to test the effect of the influence of different promoter to the expression of rtTA3.
+
This part consists with promoter pCMV can basic part rtTA3. pCMV is a strong mammalian expression promoter derived from human cytomegalovirus, usually used for routine expression, with high expression levels but may be inhibited in some cells. rtTA is a key component of Tet-On system, and rtTA3 is third generation rtTA. rtTA3 is an activation factor of pTRE. rtTA3 isn’t active when it is present alone, and it can be active when combine with doxycycline (DOX), then the complex can combine to pTRE and start the downstream transcription. When DOX get removed the rtTA3 will became no activation again. In our experiment, pCMV-rtTA3 is used to test the effect of the influence of different promoter to the expression of rtTA3.
  
 
===Source===
 
===Source===

Latest revision as of 01:20, 20 October 2019


pCMV-rtTA3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part consists with promoter pCMV can basic part rtTA3. pCMV is a strong mammalian expression promoter derived from human cytomegalovirus, usually used for routine expression, with high expression levels but may be inhibited in some cells. rtTA is a key component of Tet-On system, and rtTA3 is third generation rtTA. rtTA3 is an activation factor of pTRE. rtTA3 isn’t active when it is present alone, and it can be active when combine with doxycycline (DOX), then the complex can combine to pTRE and start the downstream transcription. When DOX get removed the rtTA3 will became no activation again. In our experiment, pCMV-rtTA3 is used to test the effect of the influence of different promoter to the expression of rtTA3.

Source

pCMV-rtTA3

References

1 Boshart M, Weber F, Jahn G, et al. A very strong enhancer is located upstream of an immediate early gene of human cytomegalovirus[J]. cell, 1985, 41(2): 521-530.
2 Xie Z, Wroblewska L, Prochazka L, et al. Multi-input RNAi-based logic circuit for identification of specific cancer cells[J]. Science, 2011, 333(6047): 1307-1311.