Difference between revisions of "Part:BBa K3165038"

 
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<partinfo>BBa_K3165038 short</partinfo>
 
<partinfo>BBa_K3165038 short</partinfo>
  
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This part places the bacteriostatic growth-inhibitory protein, Gp2 under the inducible promoter : pCpcG2.
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This part places the bacteriostatic growth-inhibitory protein, Gp2 under the inducible promoter: pCpcG2.
  
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===Usage and Biology===
 
  
 
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'><b>Sequence and Features</b></span>
 
<partinfo>BBa_K3165038 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3165038 SequenceAndFeatures</partinfo>
  
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===Usage and Biology===
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This part encodes for a bacteriostatic growth-inhibitory protein, Gp2 under an inducible promoter pCpcG2. The promoter is activated by the phosphorylated form of CcaR (a response regulator) and deactivated by dephosphorylated CcaR. the phosphorylation/dephosphorylation of CcaR depends on the light that is shone and perceived by the trans-membrane CcaS protein. <br>
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Upon light stimulation, the production of Gp2 is triggered leading to a bacteriostatic effect. On withdrawal of the light stimuli, the production of Gp2 is hindered and owing to its low half-life, the Gp2 already present in the cells is also degraded. <br>
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This part is used in association with the CcaS-CcaR system as a light-induced growth regulation system.
  
 
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<!-- Uncomment this to enable Functional Parameter display  

Revision as of 01:00, 20 October 2019


Gp2 under PcpcG2 Expression

This part places the bacteriostatic growth-inhibitory protein, Gp2 under the inducible promoter: pCpcG2.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

This part encodes for a bacteriostatic growth-inhibitory protein, Gp2 under an inducible promoter pCpcG2. The promoter is activated by the phosphorylated form of CcaR (a response regulator) and deactivated by dephosphorylated CcaR. the phosphorylation/dephosphorylation of CcaR depends on the light that is shone and perceived by the trans-membrane CcaS protein.
Upon light stimulation, the production of Gp2 is triggered leading to a bacteriostatic effect. On withdrawal of the light stimuli, the production of Gp2 is hindered and owing to its low half-life, the Gp2 already present in the cells is also degraded.
This part is used in association with the CcaS-CcaR system as a light-induced growth regulation system.