Difference between revisions of "Part:BBa K3269001"
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<partinfo>BBa_K3269001 short</partinfo>
 
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UV damage detector
 
UV damage detector
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The uvrA promoter involved in Nucleotide Excision Repair (NER) in E. coli cells allows for SOS controlled expression of sfGFP, allowing for a visual output of the amount uvrA is expressed, and therefore the amount of Damage accrued within the genome. 
  
 
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===Usage and Biology===
 
===Usage and Biology===
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The uvrA promoter allows for SOS controlled expression of GFP, allowing for a visual output of the amount uvrA is expressed, and therefore the amount of Damage occuring accrued within the genome.  during the SOS response repair mechanism when DNA is damaged through UV radiation. sfGFP is expressed in response to DNA damage.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 00:58, 20 October 2019


uvrA GFP UV Damage Sensor
UV damage detector
The uvrA promoter involved in Nucleotide Excision Repair (NER) in E. coli cells allows for SOS controlled expression of sfGFP, allowing for a visual output of the amount uvrA is expressed, and therefore the amount of Damage accrued within the genome.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 586
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 976
    Illegal BsaI site found at 1154