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Revision as of 00:48, 20 October 2019


mini-CcaS Generator

This part is used as a generator for mini-CcaS under a constitutive promoter and a strong ribosome site with a double terminator.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

CcaS protein is a cyanobacteriochrome sensor histidine kinase consisting of an N-terminal GAF domain (cGMP phosphodiesterase/ adenyl cyclase/ FhlA) bound to two PAS domains of unknown function attached to a C-terminal Histidine Kinase domain. It is the light-sensitive part of the photo-sensitive CcaS-CcaR system.
This part helps in the synthesis of CcaS protein constitutively under a strong ribosome binding site. In association with the Ho1-PcyA-CcaR gene system, this part forms the complete optogenetic CcaS-CcaR system.