Difference between revisions of "Part:BBa K3165037"

 
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<partinfo>BBa_K3165037 short</partinfo>
 
<partinfo>BBa_K3165037 short</partinfo>
  
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this part is used as a generator for mini-CcaS under a constitutive promoter and a strong ribosome site with a double terminator.
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This part is used as a generator for mini-CcaS under a constitutive promoter and a strong ribosome site with a double terminator.
  
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<!-- Add more about the biology of this part here
 
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===Usage and Biology===
 
  
 
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<partinfo>BBa_K3165037 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3165037 SequenceAndFeatures</partinfo>
  
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===Usage and Biology===
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CcaS protein is a cyanobacteriochrome sensor histidine kinase consisting of an N-terminal GAF domain (cGMP phosphodiesterase/ adenyl cyclase/ FhlA) bound to two PAS domains of unknown function attached to a C-terminal Histidine Kinase domain. It is the light-sensitive part of the photo-sensitive CcaS-CcaR system. <br>
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This part helps in the synthesis of CcaS protein constitutively under a strong ribosome binding site. In association with the Ho1-PcyA-CcaR gene system, this part forms the complete optogenetic CcaS-CcaR system.
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Revision as of 00:47, 20 October 2019


mini-CcaS Generator

This part is used as a generator for mini-CcaS under a constitutive promoter and a strong ribosome site with a double terminator.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

CcaS protein is a cyanobacteriochrome sensor histidine kinase consisting of an N-terminal GAF domain (cGMP phosphodiesterase/ adenyl cyclase/ FhlA) bound to two PAS domains of unknown function attached to a C-terminal Histidine Kinase domain. It is the light-sensitive part of the photo-sensitive CcaS-CcaR system.
This part helps in the synthesis of CcaS protein constitutively under a strong ribosome binding site. In association with the Ho1-PcyA-CcaR gene system, this part forms the complete optogenetic CcaS-CcaR system.