Difference between revisions of "Part:BBa K2957097"

 
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<partinfo>BBa_K2957017 short</partinfo>
 
<partinfo>BBa_K2957017 short</partinfo>
  
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"Contents: CMV, CCL5, Flag tag, SynthpA, with Carb resistance
+
Contents: CMV, CCL5, Flag tag, SynthpA, with Carb resistance
  
 
What is it?: A Composite part; it is a genetic circuit with CMV promoter and CCL5 gene allowing for constitutive CCL5 expression.  
 
What is it?: A Composite part; it is a genetic circuit with CMV promoter and CCL5 gene allowing for constitutive CCL5 expression.  

Revision as of 00:21, 20 October 2019


CMV, CCL5-Flag

Contents: CMV, CCL5, Flag tag, SynthpA, with Carb resistance

What is it?: A Composite part; it is a genetic circuit with CMV promoter and CCL5 gene allowing for constitutive CCL5 expression.

What does it do?: It codes for the chemokine, CCL5, which is a chemoattractant for T cells. It was important for our project in particular because of its involvement in the immune system. It has a Flag tag attached.

How to use it?: This part was used for Type IIS Cloning and transfected into HEKs. We then were able to test for secretion of CCL5. See more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>. "

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1089
    Illegal SpeI site found at 92
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 92
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1089
    Illegal SpeI site found at 92
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1089
    Illegal SpeI site found at 92
    Illegal AgeI site found at 1095
  • 1000
    COMPATIBLE WITH RFC[1000]