Difference between revisions of "Part:BBa K3165035"
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CcaR acts as a cognate response regulator for the activation of pCpcG2 promoter among activation by Histidine Kinase domain of the CcaS system. CcaR protein is constitutively produced in the cell and gets activated only when the light of specific wavelengths is shone upon it. This part produces CcaR under a constitutive promoter and a strong RBS. | CcaR acts as a cognate response regulator for the activation of pCpcG2 promoter among activation by Histidine Kinase domain of the CcaS system. CcaR protein is constitutively produced in the cell and gets activated only when the light of specific wavelengths is shone upon it. This part produces CcaR under a constitutive promoter and a strong RBS. | ||
| − | <h2> Usage <h2> | + | <h2> Usage </h2> |
This part is an essential component of the CcaSR optogenetic system stimulated by green and red light. CcaR helps in the activation of the pCpcG2 promoter when phosphorylated by the histidine kinase domain of mini-CcaS, thus regulating the protein expression. | This part is an essential component of the CcaSR optogenetic system stimulated by green and red light. CcaR helps in the activation of the pCpcG2 promoter when phosphorylated by the histidine kinase domain of mini-CcaS, thus regulating the protein expression. | ||
Revision as of 00:12, 20 October 2019
CcaR v2.0 Generator
This part is a generator for the CcaR response regulator protein under a constitutive promoter and a strong ribosome binding site with a double terminator.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
Biology
CcaR acts as a cognate response regulator for the activation of pCpcG2 promoter among activation by Histidine Kinase domain of the CcaS system. CcaR protein is constitutively produced in the cell and gets activated only when the light of specific wavelengths is shone upon it. This part produces CcaR under a constitutive promoter and a strong RBS.
Usage
This part is an essential component of the CcaSR optogenetic system stimulated by green and red light. CcaR helps in the activation of the pCpcG2 promoter when phosphorylated by the histidine kinase domain of mini-CcaS, thus regulating the protein expression.