Difference between revisions of "Part:BBa K2957015"

 
Line 3: Line 3:
 
<partinfo>BBa_K2957015 short</partinfo>
 
<partinfo>BBa_K2957015 short</partinfo>
  
−
"Contents: CMV, CCL5, SynthpA, with Carb resistance
+
<b>Contents:</b> CMV, CCL5, SynthpA, with Carb resistance
−
What is it?: A Composite part; it is a genetic circuit with CMV promoter and CCL5 gene allowing for constitutive CCL5 expression  
+
 
−
What does it do?: It codes for the chemokine, CCL5, which is a chemoattractant for T cells. It was important for our project in particular because of its involvement in the immune system.   
+
<b>What is it?:</b> A Composite part; it is a genetic circuit with CMV promoter and CCL5 gene allowing for constitutive CCL5 expression  
−
How to use it?: This part was used for Type IIS Cloning and transfected into HEKs. We then were able to test for secretion of CCL5. See more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>. "
+
 
 +
<b>What does it do?:</b> It codes for the chemokine, CCL5, which is a chemoattractant for T cells. It was important for our project in particular because of its involvement in the immune system.   
 +
 
 +
<b>How to use it?:</b> This part was used for Type IIS Cloning and transfected into HEKs. We then were able to test for secretion of CCL5. See more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>. "
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 00:00, 20 October 2019


CMV, CCL5

Contents: CMV, CCL5, SynthpA, with Carb resistance

What is it?: A Composite part; it is a genetic circuit with CMV promoter and CCL5 gene allowing for constitutive CCL5 expression

What does it do?: It codes for the chemokine, CCL5, which is a chemoattractant for T cells. It was important for our project in particular because of its involvement in the immune system.

How to use it?: This part was used for Type IIS Cloning and transfected into HEKs. We then were able to test for secretion of CCL5. See more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>. "

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1065
    Illegal SpeI site found at 92
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 92
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1065
    Illegal SpeI site found at 92
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1065
    Illegal SpeI site found at 92
    Illegal AgeI site found at 1071
  • 1000
    COMPATIBLE WITH RFC[1000]