Difference between revisions of "Part:BBa K2984001"
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This vector is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. It was shown that the putative signal sequence of the metalloprotease gametolysin (GLE) from C. reinhardtii can induce secretion (Ramos-Martinez, Fimognari, & Sakuragi, 2017). The amino acid sequence of it is MSLATRRFGAAAALLVAACVLCTAPAWA.
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This vector is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a> It was shown that the putative signal sequence of the metalloprotease gametolysin (GLE) from C. reinhardtii can induce secretion (Ramos-Martinez, Fimognari, & Sakuragi, 2017). The amino acid sequence of it is MSLATRRFGAAAALLVAACVLCTAPAWA.
 
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Revision as of 23:49, 19 October 2019


3'UTR, Secretion signal of gametolysin GLE B2-B2

This vector is a part of the Chlamy-HUB-Collection It was shown that the putative signal sequence of the metalloprotease gametolysin (GLE) from C. reinhardtii can induce secretion (Ramos-Martinez, Fimognari, & Sakuragi, 2017). The amino acid sequence of it is MSLATRRFGAAAALLVAACVLCTAPAWA.

This part was designed to be used with the MoClo standard and has B2-B2 overhangs.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization

Plate_L0-GLE_B2-B2_E.coli
Fig.1 - Successful transformation of L0-GLE_B2-B2 into E. coli.


References

  1. Ramos‐Martinez, E. M., Fimognari, L., & Sakuragi, Y. (2017). High‐yield secretion of recombinant proteins from the microalga Chlamydomonas reinhardtii. Plant biotechnology journal, 15(9), 1214-1224.