Difference between revisions of "Part:BBa K3165033"

Revision as of 23:44, 19 October 2019

RBS + ECFP

The codon optimised engineered cyan fluorescent protein (ECFP) is placed along with a ribosome binding site.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Usage and Biology

Biology

Engineered Cyan Fluorescent protein is a commonly used reporter gene for biological studies. Owing to its photostability, sufficiently high lifespan and relatively low maturation times, ECFP is a popular choice as a fluorescent marker. ECFP paired with an RBS (BBa_B0034) is used to generate a translational unit for the production of the fluorophore.

Usage

This part is used as a reporter unit under any suitable promoter. Under a photo-sensitive promoter system, this part can be used to characterise the functioning of the inducible system based on fluorescence measurements.

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 The codon optimised engineered cyan fluorescent protein (ECFP) is placed along with a ribosome binding site.
-<!-- Add more about the biology of this part here
-===Usage and Biology===
 <!-- -->
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 <partinfo>BBa_K3165033 SequenceAndFeatures</partinfo>
+===Usage and Biology===
+<h2> Biology </h2>
+Engineered Cyan Fluorescent protein is a commonly used reporter gene for biological studies. Owing to its photostability, sufficiently high lifespan and relatively low maturation times, ECFP is a popular choice as a fluorescent marker.
+ECFP paired with an RBS (BBa_B0034) is used to generate a translational unit for the production of the fluorophore.
+<h2> Usage </h2>
+This part is used as a reporter unit under any suitable promoter. Under a photo-sensitive promoter system, this part can be used to characterise the functioning of the inducible system based on fluorescence measurements.
 <!-- Uncomment this to enable Functional Parameter display