Difference between revisions of "Part:BBa K112001:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K112001 short</partinfo> | <partinfo>BBa_K112001 short</partinfo> | ||
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===Source=== | ===Source=== | ||
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PCR bx3/bx2 on Bjh1301 (685 bp, EcoRI/BamHI/DpnI) | PCR bx3/bx2 on Bjh1301 (685 bp, EcoRI/BamHI/DpnI) | ||
Sub into pBca1256 (EcoRI/BamHI) | Sub into pBca1256 (EcoRI/BamHI) | ||
Revision as of 18:10, 21 October 2008
Xis from bacteriophage lambda, assembly standard 21
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Source
PCR bx3/bx2 on Bjh1301 (685 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112001
----------------------------------------
bx3 Forward biobricking of {<T4 Holin!}
ccagtGAATTCatgAGATCTgcagcacctagaatatcattttcgccc
bx2 Reverse biobricking of {<T4 Holin!}
cgttaGGATCCttatttagcccttcctaatattctggccgcc