Difference between revisions of "Part:BBa K3183201"

Revision as of 23:27, 19 October 2019

Truncated CD27L 1-179 with 6-His and SpyTag

This part contains CD27L fused to an N-Terminal 6-His Tag and SpyTag for purification, oligomerisation, and other SpyCatcher applications. This endolysin has previously been shown to be specific to C. difficile. This endolysin works by cleaving residues¹ (Fig. 1), breaking the cell's peptidoglycan and compromising the structural integrity of its cell wall. Two X-ray crystal structures were solved for the N² and C-terminal domains³. These are joined by a linker, which we've added ourselves (Figure 2).

Figure 2: X-ray Crystal Structure of CD27L (PDB codes 3QAY & 4CU5

Figure 1: CD27L Catalytic Reaction.

Sequence and Features