Difference between revisions of "Part:BBa K3105681:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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For this design, overhangs complementary to the pPICZαB vector (easy select pichia expression kit from invitrogen) were added by PCR. This was then assembled into the expression vector generating the circuit in this part
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For this design, overhangs complementary to the pPICZ&#945;B vector (Invitrogen easy select <I>Pichia</I> expression kit) were added by PCR. This was then assembled into the expression vector generating the circuit in this part
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===Source===
 
===Source===

Revision as of 23:22, 19 October 2019


Expression construct LiP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1183
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2453
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For this design, overhangs complementary to the pPICZαB vector (Invitrogen easy select Pichia expression kit) were added by PCR. This was then assembled into the expression vector generating the circuit in this part

Source

see basic parts

References