Difference between revisions of "Part:BBa K2984073"

Latest revision as of 23:21, 19 October 2019

3x HA-Tag for protein detection, isolation, purification

A popular in vitro purification und detection tag is the Human influenza hemagglutinin (HA) tag (Wilson et al., 1984). It is a glycoprotein which is required for infectivity of the virus. The tag itself is a small segment of the virus commonly used as an epitope tag in gene expression vectors. Three copies of this tag attached to the desired protein will increase the affinity towards the specific HA-antibodies, therefore, eliminating the production of protein-specific antibodies. The amino acid sequence is YPYDVPDYA. The amino acid sequence was also translated to base triplets in a codon-optimized way for C. reinhardtii.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

MoClo Overhangs

This part with B4-B5 MoClo overhangs can be found here.

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 <partinfo>BBa_K2984073 short</partinfo>
-A popular in vitro purification und detection tag is the Human influenza hemagglutinin (HA) tag (Wilson et al., 1984). It’s a glycoprotein which is required for infectivity of the virus. The tag itself is a small segment of the virus commonly used as an epitope tag in gene expression vectors. Three copies of this tag attached to the desired protein will increase the affinity towards the specific HA-antibodies therefore eliminating the production of protein specific antibodies. The amino acid sequence is YPYDVPDYA. The amino acid sequence was also translated to base triplets in a codon optimized way for C. reinhardtii.
+A popular in vitro purification und detection tag is the Human influenza hemagglutinin (HA) tag (Wilson et al., 1984). It is a glycoprotein which is required for infectivity of the virus. The tag itself is a small segment of the virus commonly used as an epitope tag in gene expression vectors. Three copies of this tag attached to the desired protein will increase the affinity towards the specific HA-antibodies, therefore, eliminating the production of protein-specific antibodies. The amino acid sequence is YPYDVPDYA. The amino acid sequence was also translated to base triplets in a codon-optimized way for <i>C. reinhardtii</i>.
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