Difference between revisions of "Part:BBa K3165032"
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This part incorporates the CcaR encoding gene under a strong ribosome binding site [sRBS(250)]. | This part incorporates the CcaR encoding gene under a strong ribosome binding site [sRBS(250)]. | ||
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− | <span class='h3bb'>Sequence and Features</span> | + | <span class='h3bb'><b<Sequence and Features</b></span> |
<partinfo>BBa_K3165032 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3165032 SequenceAndFeatures</partinfo> | ||
+ | ===Usage and Biology=== | ||
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+ | <h2> Biology </h2> | ||
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+ | The CcaR encoding sequence is attached to a strong ribosome binding site [sRBS (250)] for the constitutive production of the CcaR protein in the cell under normal metabolic conditions. | ||
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+ | <h3> Usage </h3> | ||
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+ | This part is used as an essential component of the green light-sensitive CcaSR system. In association with the BioBrick encoding for the CcaS protein the green light-inducible protein expression system is established. | ||
<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display |
Revision as of 23:07, 19 October 2019
sRBS + CcaR v2.0
This part incorporates the CcaR encoding gene under a strong ribosome binding site [sRBS(250)].
<b<Sequence and Features</b>
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
Biology
The CcaR encoding sequence is attached to a strong ribosome binding site [sRBS (250)] for the constitutive production of the CcaR protein in the cell under normal metabolic conditions.
Usage
This part is used as an essential component of the green light-sensitive CcaSR system. In association with the BioBrick encoding for the CcaS protein the green light-inducible protein expression system is established.