Difference between revisions of "Part:BBa K3105677"
| Line 3: | Line 3: | ||
<partinfo>BBa_K3105677 short</partinfo> | <partinfo>BBa_K3105677 short</partinfo> | ||
| − | Expression circuit for HRP and eGFP in Pichia pastoris. HRP will be secreted due to the addition of the α-factor secretion signal and eGFP will remain in the cell. The 2A-selfcleaving peptide will lead to cleavage of the polypeptide, achieving separation of HRP from eGFP. | + | Expression circuit for HRP and eGFP in <I>Pichia pastoris</I>. HRP will be secreted due to the addition of the α-factor secretion signal and eGFP will remain in the cell. The 2A-selfcleaving peptide will lead to cleavage of the polypeptide, achieving separation of HRP from eGFP. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 23:06, 19 October 2019
Expression construct HRP-2A-eGFP
Expression circuit for HRP and eGFP in Pichia pastoris. HRP will be secreted due to the addition of the α-factor secretion signal and eGFP will remain in the cell. The 2A-selfcleaving peptide will lead to cleavage of the polypeptide, achieving separation of HRP from eGFP.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2086
Illegal XhoI site found at 1183 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2167
Illegal AgeI site found at 3176 - 1000COMPATIBLE WITH RFC[1000]