Difference between revisions of "Part:BBa K137017"
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| − | Galactose oxidase is a fungal enzyme that uses molecular oxygen to oxidizes galactose and several other primary alcohols to the corresponding aldehyde, while also producing hydrogen peroxide. This particular galaxtose oxidase is not wild type. It has been optimized through directed evolution by the Arnold lab at CalTech to be functionally expressed in vivo in E. coli and to be thermostable. (Sun L, Petrounia IP, Yagasaki M, Bandara G, and Arnold FH. Expression and stabilization of galactose oxidase in Escherichia coli by directed evolution. Protein Eng 2001 Sep; 14(9) 699-704. pmid:11707617). This part has undergone two rounds of site directed mutagenesis to remove one EcoRI and one PstI site. In our hands, it is functional towards galactose in vivo. | + | Galactose oxidase is a fungal enzyme that uses molecular oxygen to oxidizes galactose and several other primary alcohols to the corresponding aldehyde, while also producing hydrogen peroxide. This particular galaxtose oxidase is not wild type. It has been optimized through directed evolution by the Arnold lab at CalTech to be functionally expressed in vivo in E. coli and to be thermostable. (Sun L, Petrounia IP, Yagasaki M, Bandara G, and Arnold FH. Expression and stabilization of galactose oxidase in Escherichia coli by directed evolution. Protein Eng 2001 Sep; 14(9) 699-704. pmid:11707617). This part has undergone two rounds of site directed mutagenesis to remove one EcoRI and one PstI site. |
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| + | In our hands, it is functional towards galactose in vitro, but not in vivo. | ||
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Latest revision as of 16:46, 21 October 2008
Galactose Oxidase
Galactose oxidase is a fungal enzyme that uses molecular oxygen to oxidizes galactose and several other primary alcohols to the corresponding aldehyde, while also producing hydrogen peroxide. This particular galaxtose oxidase is not wild type. It has been optimized through directed evolution by the Arnold lab at CalTech to be functionally expressed in vivo in E. coli and to be thermostable. (Sun L, Petrounia IP, Yagasaki M, Bandara G, and Arnold FH. Expression and stabilization of galactose oxidase in Escherichia coli by directed evolution. Protein Eng 2001 Sep; 14(9) 699-704. pmid:11707617). This part has undergone two rounds of site directed mutagenesis to remove one EcoRI and one PstI site.
In our hands, it is functional towards galactose in vitro, but not in vivo.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1294
Illegal NotI site found at 517 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1424
Illegal BamHI site found at 144
Illegal BamHI site found at 589
Illegal BamHI site found at 624
Illegal BamHI site found at 782 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]