Difference between revisions of "Part:BBa K2957031"
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<b>Contents:</b> TRE promoter with CCL5 Gene, Carb resistance
 
<b>Contents:</b> TRE promoter with CCL5 Gene, Carb resistance
 +
<br>
 
<b>What is it?:</b> A Composite part; we call it a pL1  
 
<b>What is it?:</b> A Composite part; we call it a pL1  
 +
<br>
 
<b>What does it do?:</b> It codes for the chemokine, CCL5, which is involved in immune response. It was important for our project in particular because of its involvement in the immune system and is known to signal chemotaxis of T-cells.  
 
<b>What does it do?:</b> It codes for the chemokine, CCL5, which is involved in immune response. It was important for our project in particular because of its involvement in the immune system and is known to signal chemotaxis of T-cells.  
 +
<br>
 
<b>How to use it?:</b> This part was created with Type IIS Cloning and transfected into HEKs. We tested it with Jurkats. See more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>.
 
<b>How to use it?:</b> This part was created with Type IIS Cloning and transfected into HEKs. We tested it with Jurkats. See more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>.
  

Revision as of 22:49, 19 October 2019


TRE, CCL5

Contents: TRE promoter with CCL5 Gene, Carb resistance
What is it?: A Composite part; we call it a pL1
What does it do?: It codes for the chemokine, CCL5, which is involved in immune response. It was important for our project in particular because of its involvement in the immune system and is known to signal chemotaxis of T-cells.
How to use it?: This part was created with Type IIS Cloning and transfected into HEKs. We tested it with Jurkats. See more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2943
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2943
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2943
    Illegal NgoMIV site found at 1479
    Illegal AgeI site found at 2949
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2623
    Illegal BsaI.rc site found at 2921
    Illegal SapI site found at 402
    Illegal SapI.rc site found at 2560