Difference between revisions of "Part:BBa K2957032"

 
 
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<b>Contents:</b> TRE promoter with CCL5 gene and Flag tag, Carb resistance
 
<b>Contents:</b> TRE promoter with CCL5 gene and Flag tag, Carb resistance
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<br>
 
<b>What is it?:</b> A Composite part; we call it a pL1  
 
<b>What is it?:</b> A Composite part; we call it a pL1  
 +
<br>
 
<b>What does it do?:</b> It codes for the chemokine, CCL5, which is involved in immune response. It was important for our project in particular because of its involvement in the immune system and is known to signal chemotaxis of T-cells.  
 
<b>What does it do?:</b> It codes for the chemokine, CCL5, which is involved in immune response. It was important for our project in particular because of its involvement in the immune system and is known to signal chemotaxis of T-cells.  
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<b>How to use it?:</b> This part was created with Type IIS Cloning and transfected into HEKs. We tested it with Jurkats. See more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>.
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<br>
 +
<b>How to use it?:</b> This part was created with Type IIS Cloning and transfected into HEKs. We tested it with Jurkats. See more on the Parts Overview Page or MIT iGEM 2019 wiki: https://2019.igem.org/Team:MIT/Parts
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 22:48, 19 October 2019


TRE, CCL5-Flag

Contents: TRE promoter with CCL5 gene and Flag tag, Carb resistance
What is it?: A Composite part; we call it a pL1
What does it do?: It codes for the chemokine, CCL5, which is involved in immune response. It was important for our project in particular because of its involvement in the immune system and is known to signal chemotaxis of T-cells.
How to use it?: This part was created with Type IIS Cloning and transfected into HEKs. We tested it with Jurkats. See more on the Parts Overview Page or MIT iGEM 2019 wiki: https://2019.igem.org/Team:MIT/Parts

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2967
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2967
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2967
    Illegal NgoMIV site found at 1479
    Illegal AgeI site found at 2973
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2623
    Illegal BsaI.rc site found at 2945
    Illegal SapI site found at 402
    Illegal SapI.rc site found at 2560