Difference between revisions of "Part:BBa K2957031"

 
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<b>What is it?:</b> A Composite part; we call it a pL1  
 
<b>What is it?:</b> A Composite part; we call it a pL1  
 
<b>What does it do?:</b> It codes for the chemokine, CCL5, which is involved in immune response. It was important for our project in particular because of its involvement in the immune system and is known to signal chemotaxis of T-cells.  
 
<b>What does it do?:</b> It codes for the chemokine, CCL5, which is involved in immune response. It was important for our project in particular because of its involvement in the immune system and is known to signal chemotaxis of T-cells.  
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<b>How to use it?:</b> You can use this part for Type IIS Cloning. It has BsaI cut sites that allows for this. With other Basic parts like pL0-P (promoter/regulatory sites), pL0-T (terminator part), pL0-5.2/pL0-3 (Inert pieces that form the UTR regions around the genetic circuit), you can use this part to make a composite part. So the pL0's are placed in a given backbone depending on the kind of basic part it is. In this case, we have the CDS/gene part which is in a pL0-G backbone. See more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>.
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<b>How to use it?:</b> This part was created with Type IIS Cloning and transfected into HEKs. We tested it with Jurkats. See more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 22:44, 19 October 2019


TRE, CCL5

Contents: TRE promoter with CCL5 Gene, Carb resistance What is it?: A Composite part; we call it a pL1 What does it do?: It codes for the chemokine, CCL5, which is involved in immune response. It was important for our project in particular because of its involvement in the immune system and is known to signal chemotaxis of T-cells. How to use it?: This part was created with Type IIS Cloning and transfected into HEKs. We tested it with Jurkats. See more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2943
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2943
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2943
    Illegal NgoMIV site found at 1479
    Illegal AgeI site found at 2949
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2623
    Illegal BsaI.rc site found at 2921
    Illegal SapI site found at 402
    Illegal SapI.rc site found at 2560