Difference between revisions of "Part:BBa K2984043"

Revision as of 22:41, 19 October 2019

L1c-PsaD-MHETase-3xHA-RbcS2

This vector is a part of the Chlamy-HUB-Collection. This part is composed of the PsaD promoter, a B2-B2 linker, the MHETase enzyme, kindly provided by the iGEM TU Kaiserslautern 2019 team, a 3xHA-tag, and the Rbcs2 terminator. The part can be used to express the MHETase enzyme in C. reinhardtii. The 3xHA tag can be used to detect and purify the MHETase.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 1609
Illegal PstI site found at 1933
Illegal PstI site found at 2276
Illegal PstI site found at 3086
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 1609
Illegal PstI site found at 1933
Illegal PstI site found at 2276
Illegal PstI site found at 3086
Illegal NotI site found at 1944
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 2854
Illegal BamHI site found at 4
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 1609
Illegal PstI site found at 1933
Illegal PstI site found at 2276
Illegal PstI site found at 3086
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 1609
Illegal PstI site found at 1933
Illegal PstI site found at 2276
Illegal PstI site found at 3086
Illegal NgoMIV site found at 1542
Illegal NgoMIV site found at 2003
Illegal NgoMIV site found at 2033
Illegal NgoMIV site found at 2348
Illegal NgoMIV site found at 2366
Illegal NgoMIV site found at 2402
Illegal NgoMIV site found at 3045
1000
COMPATIBLE WITH RFC[1000]

Characterization

colonies_total — Fig.1 - Image of a successful transformation in *E. coli* after ligation of the construct. The construct can then be isolated from *E. coli* to be transformed in *C. reinhardtii*

@@ Line 4: / Line 4: @@
 <html>
-This vector is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. This part is composed of the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984008">PsaD promoter</a>, a <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984034">B2-B2 linker</a>, the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K3002037">MHETase enzyme</a>, kindly provided by the iGEM TU Kaiserslautern 2019 team, a <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K3002015">3xHA-tag</a>, and the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984018">Rbcs2 terminator</a>. The part can be used to express the MHETase enzyme in C. reinhardtii. The 3xHA tag can be used to detect and purify the MHETase.
+This vector is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. This part is composed of the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984008">PsaD promoter</a>, a <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984034">B2-B2 linker</a>, the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K3002037">MHETase enzyme</a>, kindly provided by the iGEM TU Kaiserslautern 2019 team, a <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K3002015">3xHA-tag</a>, and the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984018">Rbcs2 terminator</a>. The part can be used to express the MHETase enzyme in <i>C. reinhardtii</i>. The 3xHA tag can be used to detect and purify the MHETase.
 </html>
@@ Line 18: / Line 18: @@
 <figure>
 <img src="https://2019.igem.org/wiki/images/8/8a/T--Humboldt_Berlin--Konstruk_12.jpg" alt="colonies_total" width="500">
-<figcaption>Fig.1 - Image of a successful transformation in E.coli after ligation of the construct. The construct can then be isolated from E. coli to be transformed in C. reinhardtii</figcaption>
+<figcaption>Fig.1 - Image of a successful transformation in <i>E. coli</i> after ligation of the construct. The construct can then be isolated from <i>E. coli</i> to be transformed in <i>C. reinhardtii</i></figcaption>
 </figure>
 </html>