Difference between revisions of "Part:BBa K2957057"
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Contents: hEF1a | Contents: hEF1a | ||
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What is it?: A basic part; it is a promoter and allows for constitutive expression of a gene. | What is it?: A basic part; it is a promoter and allows for constitutive expression of a gene. | ||
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What does it do?: This promoter expresses continually, and was put in our genetic circuits to continually produce the chemokines we were testing. | What does it do?: This promoter expresses continually, and was put in our genetic circuits to continually produce the chemokines we were testing. | ||
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How to use it?: This part was used in Type IIS Cloning when we made our composite parts. See more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>. | How to use it?: This part was used in Type IIS Cloning when we made our composite parts. See more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>. | ||
Latest revision as of 21:43, 19 October 2019
hEF1a
Contents: hEF1a
What is it?: A basic part; it is a promoter and allows for constitutive expression of a gene.
What does it do?: This promoter expresses continually, and was put in our genetic circuits to continually produce the chemokines we were testing.
How to use it?: This part was used in Type IIS Cloning when we made our composite parts. See more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 81
- 1000COMPATIBLE WITH RFC[1000]