Difference between revisions of "Part:BBa K112101:Design"

 
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__NOTOC__
 
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<partinfo>BBa_K112101 short</partinfo>
 
<partinfo>BBa_K112101 short</partinfo>
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mv004 Reverse Biobricking of Cre>
 
mv004 Reverse Biobricking of Cre>
 
cgttaGGATCCatcgccatcttccagcaggcg
 
cgttaGGATCCatcgccatcttccagcaggcg
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Product is pBca1256-K112101
 
Product is pBca1256-K112101
 
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===References===
 
===References===

Latest revision as of 10:48, 21 October 2008

Cre enzyme without stop codon


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 42
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites. 

PCR mv003/mv004 on pBca9145-Bca1010    (1060bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112101
-----------------------------------------------
mv003	Forward Biobricking of Cre>
ccataGAATTCatgAGATCTatgtccaatttactgaccgtac
mv004	Reverse Biobricking of Cre>
cgttaGGATCCatcgccatcttccagcaggcg


Source

PCR mv003/mv004 on pBca9145-Bca1010    (1060bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112101

References