Difference between revisions of "Part:BBa K3127995"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | Our team has optimized the BBa_S04615 part. This part consists of the promoter B0034 and the BBa_K592001. | ||
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| + | In 2018, the SDU-CHINA team optimized the K592001 part to reduce its leakage under the red light. We have improved its promoters this year, which has further bettered the performance of this component. | ||
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| + | The component BBa_K2627000 proved the progress of the optimized CcaS by sufficient characterization, so we directly replaced K592001 in BBa_S04615 with the optimized K2627000 when characterizing this component. In 2014, Schmidl S R , Sheth R U, Wu A, et al. optimized CcaS by constructing an RBS library. This document has fully proven that the performance of components with optimized RBS is better than S04615. In 2019, our team SDU-CHINA has further improved the components based on them. We have introduced site-directed mutagenesis to the improved promoter, further bettering the performance based on all previous studies. | ||
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Revision as of 20:19, 19 October 2019
Advanced RBS for CcaS
Improved Construction intermediate
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 623
Illegal NheI site found at 680 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1396
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]