Difference between revisions of "Part:BBa K2929002"

 
 
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5' NcoI and 3' BamHI cut sites.  
 
5' NcoI and 3' BamHI cut sites.  
  
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===Usage and Biology===
 
===Usage and Biology===
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<p> The Human IgE CH3 Domain expresses well at 25°C in SoluBL21 cells in a pEHISTEV vector, with 0.2 mM IPTG induction overnight, but the expressed protein is mostly insoluble based on small-scale expression tests.</p>
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<img src="https://2019.igem.org/wiki/images/3/30/T--St_Andrews--Antibody_13_Expression_Test.jpg
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<p style="text-align:center;"><font size=-2>Figure 1. Human IgE wild-type expression, showing clear bands corresponding to insoluble recombinant protein for the induced fraction. Soluble recombinant protein expression is not visible on this gel.</p>
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Latest revision as of 20:02, 19 October 2019


Human IgE CH3 Domain

CH3 Domain of the Human IgE antibody. Codons optimised for Escherichia coli.

5' NcoI and 3' BamHI cut sites.

Usage and Biology

The Human IgE CH3 Domain expresses well at 25°C in SoluBL21 cells in a pEHISTEV vector, with 0.2 mM IPTG induction overnight, but the expressed protein is mostly insoluble based on small-scale expression tests.

Figure 1. Human IgE wild-type expression, showing clear bands corresponding to insoluble recombinant protein for the induced fraction. Soluble recombinant protein expression is not visible on this gel.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 334
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]