Difference between revisions of "Part:BBa K3009012"

Line 11: Line 11:
 
<html>
 
<html>
 
<figure>
 
<figure>
<img style="width:50%" src="https://parts.igem.org/File:T--Freiburg--racemase_pruification.png">
+
<img style="width:50%;" src="https://parts.igem.org/File:T--Freiburg--racemase_pruification.png">
 
</figure>
 
</figure>
 
</html>
 
</html>

Revision as of 19:48, 19 October 2019


Aspartate Racemase (PhAspR)

Racemases exist in many organisms, especially bacteria, and convert L- to D-amino acids for different purposes, like building the bacterial cell wall or using D-amino acids as a neurotransmitter. The aspartate racemase is consequently converting L-aspartate to D-aspartate.


We purified it via his-tag purification to perform further experiments.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 363
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]