Difference between revisions of "Part:BBa K3009012"
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Racemases exist in many organisms, especially bacteria, and convert L- to D-amino acids for different purposes, like building the bacterial cell wall or using D-amino acids as a neurotransmitter. | Racemases exist in many organisms, especially bacteria, and convert L- to D-amino acids for different purposes, like building the bacterial cell wall or using D-amino acids as a neurotransmitter. | ||
The aspartate racemase is consequently converting L-aspartate to D-aspartate. | The aspartate racemase is consequently converting L-aspartate to D-aspartate. | ||
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+ | We purified it via his-tag purification to perform further experiments. | ||
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Revision as of 19:40, 19 October 2019
Aspartate Racemase (PhAspR)
Racemases exist in many organisms, especially bacteria, and convert L- to D-amino acids for different purposes, like building the bacterial cell wall or using D-amino acids as a neurotransmitter. The aspartate racemase is consequently converting L-aspartate to D-aspartate.
We purified it via his-tag purification to perform further experiments.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 363
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]