Difference between revisions of "Part:BBa K3179000"

Revision as of 19:35, 19 October 2019

miR663b-target

MiRNA 663b-target is constructed bearing four repeats of miRNA 663b, and each repeat added with ATG. Extra bases which can be devisable by three were added in order to separate ATGs.
This part, four repeating sequences complementary to miRNA663b on an mRNA, is a 4X miRNA target designed to target miRNA663b. When miRNA663b reaches a certain concentration in the cell, it would combine with miR663b-target and suppress the translation of mRNA. According to our design, miR663b-target is designed to control the translation of mRNA containing response parts.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K3179000 short</partinfo>
-MiRNA 663b-target is constructed bearing four repeats of miRNA 663b, and each repeat added with ATG. Extra bases  which can be devisable by three were added in order to separate ATGs.
+MiRNA 663b-target is constructed bearing four repeats of miRNA 663b, and each repeat added with ATG. Extra bases  which can be devisable by three were added in order to separate ATGs.<br style="clear: both" />This part, four repeating sequences complementary to miRNA663b on an mRNA, is a 4X miRNA target designed to target miRNA663b. When miRNA663b reaches a certain concentration in the cell, it would combine with miR663b-target and suppress the translation of mRNA. According to our design, miR663b-target is designed to control the translation of mRNA containing response parts.
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