Difference between revisions of "Part:BBa K2984073"

 
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A popular in vitro purification und detection tag is the Human influenza hemagglutinin (HA) tag (Wilson et al., 1984). It’s a glycoprotein which is required for infectivity of the virus. The tag itself is a small segment of the virus commonly used as an epitope tag in gene expression vectors. Three copies of this tag attached to the desired protein will increase the affinity towards the specific HA-antibodies therefore eliminating the production of protein specific antibodies. The amino acid sequence is YPYDVPDYA. The amino acid sequence was also translated to base triplets in a codon optimized way for C. reinhardtii.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2984073 SequenceAndFeatures</partinfo>
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===MoClo Overhangs===
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<p>This part with B4-B5 MoClo overhangs can be found <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984015">here</a>.</p>
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Revision as of 19:24, 19 October 2019


3x HA-Tag for protein detection, isolation, purification

A popular in vitro purification und detection tag is the Human influenza hemagglutinin (HA) tag (Wilson et al., 1984). It’s a glycoprotein which is required for infectivity of the virus. The tag itself is a small segment of the virus commonly used as an epitope tag in gene expression vectors. Three copies of this tag attached to the desired protein will increase the affinity towards the specific HA-antibodies therefore eliminating the production of protein specific antibodies. The amino acid sequence is YPYDVPDYA. The amino acid sequence was also translated to base triplets in a codon optimized way for C. reinhardtii.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


MoClo Overhangs

This part with B4-B5 MoClo overhangs can be found here.