Difference between revisions of "Part:BBa K3142012"
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<partinfo>BBa_K3142012 parameters</partinfo> | <partinfo>BBa_K3142012 parameters</partinfo> | ||
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| + | ==SZPT-China== | ||
| + | To ensure that the transgenic bacteria are not harmful to the environment, we constructed the autolytic enzyme gene acmA of the lactic acid bacteria downstream of the glucose starvation promoter. The results of the construction of the recombinant plasmid pMG36e-pα-crp-acmA named pMG36e-P-a are shown in Fig. 18. The results of LAB MG1363 and LAB MG1363 with pMG36e-p - a after induction of recombinant bacteria by different glucose concentrations are shown in Fig. 18. The results showed that when the concentration of glucose in the environment was less than 0.05%, the growth of recombinant bacteria was significantly inhibited. | ||
| + | [[file:p-a.png|center|500px]] | ||
Revision as of 19:24, 19 October 2019
Glucose starvation promoter（PT-αcrp）
what it is：Glucose starved promoter
what it does：Response to glucose starvation
how to use it in their projects：Initiating the autolysis gene expressing lactic acid bacteria in response to glucose starvation
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 26
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 26
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 26
Illegal BamHI site found at 1 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 26
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 26
- 1000COMPATIBLE WITH RFC[1000]
SZPT-China
To ensure that the transgenic bacteria are not harmful to the environment, we constructed the autolytic enzyme gene acmA of the lactic acid bacteria downstream of the glucose starvation promoter. The results of the construction of the recombinant plasmid pMG36e-pα-crp-acmA named pMG36e-P-a are shown in Fig. 18. The results of LAB MG1363 and LAB MG1363 with pMG36e-p - a after induction of recombinant bacteria by different glucose concentrations are shown in Fig. 18. The results showed that when the concentration of glucose in the environment was less than 0.05%, the growth of recombinant bacteria was significantly inhibited.
