Difference between revisions of "Part:BBa K3286100:Design"
(→Source) |
|||
| Line 13: | Line 13: | ||
===Source=== | ===Source=== | ||
| − | + | Parts amplified from plasmid present at our university | |
===References=== | ===References=== | ||
Revision as of 18:59, 19 October 2019
Expression construct for proteins in E. coli BL21DE3
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Strep tag on the N-terminus, with the TEV cleavage site in between, which can be cut using TEV protease. This will yield the recombinant protein.
Source
Parts amplified from plasmid present at our university