Difference between revisions of "Part:BBa K2960000"
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<partinfo>BBa_K2960000 short</partinfo> | <partinfo>BBa_K2960000 short</partinfo> | ||
| − | <img class="standard-page-content-image" style = "height: | + | <img class="standard-page-content-image" style = "height: 100px; width: 175px" src="https://2019.igem.org/wiki/images/7/76/T--Cornell--Translocase.png"> |
This aptamer was used to create a rapid colorimetric microcystin-detection sensor. Gold nanoparticles (AuNPs) were adsorbed onto the aptamer surface. Normally, in the presence of NaCl, AuNPs will aggregate, shifting the solution color from red to blue. [Show picture] Adsorption onto the aptamer prevents this from happening. However, in the presence of microcystin, the aptamer dissociates from the AuNPs. Thus, they will aggregate when induced by salt, resulting in a shift in the absorption spectra of the solution. We found there to be a log-linear decrease in the absorption at 320 nm. | This aptamer was used to create a rapid colorimetric microcystin-detection sensor. Gold nanoparticles (AuNPs) were adsorbed onto the aptamer surface. Normally, in the presence of NaCl, AuNPs will aggregate, shifting the solution color from red to blue. [Show picture] Adsorption onto the aptamer prevents this from happening. However, in the presence of microcystin, the aptamer dissociates from the AuNPs. Thus, they will aggregate when induced by salt, resulting in a shift in the absorption spectra of the solution. We found there to be a log-linear decrease in the absorption at 320 nm. | ||
Revision as of 18:58, 19 October 2019
ssDNA aptamer which binds to microcystin-LR
<img class="standard-page-content-image" style = "height: 100px; width: 175px" src="
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This aptamer was used to create a rapid colorimetric microcystin-detection sensor. Gold nanoparticles (AuNPs) were adsorbed onto the aptamer surface. Normally, in the presence of NaCl, AuNPs will aggregate, shifting the solution color from red to blue. [Show picture] Adsorption onto the aptamer prevents this from happening. However, in the presence of microcystin, the aptamer dissociates from the AuNPs. Thus, they will aggregate when induced by salt, resulting in a shift in the absorption spectra of the solution. We found there to be a log-linear decrease in the absorption at 320 nm.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]