Difference between revisions of "Part:BBa K3113302"
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<partinfo>BBa_K3113302 short</partinfo>
 
<partinfo>BBa_K3113302 short</partinfo>
  
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This plasmid codes for the coat protein of HIV fused to a split luciferase fused to a synthetic L7Ae. This construct forms virus-like particles which can be detected via a split luciferase assay. It is used to target fluc specifically into exosomes via L7Ae-C/Dbox connection.
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This plasmid codes for the coat protein of HIV fused to a split luciferase fused to a synthetic L7Ae. This construct forms virus-like particles which can be detected via a split luciferase assay. It is used to target FLuc mRNA specifically into virus-like particles via L7Ae-C/Dbox interaction.
  
  

Revision as of 18:50, 19 October 2019


pCAG_Gag-HiBiT-L7Ae

This plasmid codes for the coat protein of HIV fused to a split luciferase fused to a synthetic L7Ae. This construct forms virus-like particles which can be detected via a split luciferase assay. It is used to target FLuc mRNA specifically into virus-like particles via L7Ae-C/Dbox interaction.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3019
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2319