Difference between revisions of "Part:BBa K2969027"

 
 
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sfGFP-Tyr151 is transformed from the reporting part sfGFP. The tyrosine residue at 151 site is mutated to TAG codon.
 
sfGFP-Tyr151 is transformed from the reporting part sfGFP. The tyrosine residue at 151 site is mutated to TAG codon.
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<h2>Characterization</h2>
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<p>We inserted different number of TAG codon to sfGFP at different site to see the effect of TAG codon to the expression of sfGFP. Figure 1 shows that TAG codon can inhibit the transcription of sfGFP effectively.
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<div>[[File:T--UCAS-China--sfGFP-nTAG.png|700px|thumb|center|<b>Figure 1:</b>The fluorescence of sfGEP inserted different number of TAG codon]]
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 18:43, 19 October 2019


sfGFP-Tyr151

sfGFP-Tyr151 is transformed from the reporting part sfGFP. The tyrosine residue at 151 site is mutated to TAG codon.


Characterization

We inserted different number of TAG codon to sfGFP at different site to see the effect of TAG codon to the expression of sfGFP. Figure 1 shows that TAG codon can inhibit the transcription of sfGFP effectively.

Figure 1:The fluorescence of sfGEP inserted different number of TAG codon


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]