Difference between revisions of "Part:BBa K3187020"
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<partinfo>BBa_K3187020 short</partinfo>
 
<partinfo>BBa_K3187020 short</partinfo>
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<html>
  
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                <h3>Profile</h3>
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                <table style=“width:80%“>
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                <tr>
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                <td><b>Name</b></td>
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                <td>Tobacco Etch Virus (TEV) Protease Cleavage Site</td>
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                </tr>
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                <tr>
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                <td><b>Base pairs</b></td>
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                <td>21</td>
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                </tr>
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                <tr>
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                <td><b>Molecular weight</b></td>
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                <td>871 Da</td>
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                </tr>
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                <tr>
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                <td><b>Origin</b></td>
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                <td>Tobacco Etch Virus (TEV) </td>
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                </tr>
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                <tr>
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                <td><b>Properties</b></td>
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                <td> recognition site for the TEV protease</td>
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                </tr>
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                </table>
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</html>
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===Usage and Biology===
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<html>               
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                <p>
 
This part includes the TVMV Protease cleavage site. TVMV cleaves the sequence ETVRFQS.
 
This part includes the TVMV Protease cleavage site. TVMV cleaves the sequence ETVRFQS.
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We are using this recognition site to cut our protein in front of its polyG-tag so it has a C-terminal polyG-tag that can be used in a Sortase A reaction.
  
 
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Revision as of 18:09, 19 October 2019


Tobacco Vein Mottling Virus Protease Cleavage Site

Profile

Name Tobacco Etch Virus (TEV) Protease Cleavage Site
Base pairs 21
Molecular weight 871 Da
Origin Tobacco Etch Virus (TEV)
Properties recognition site for the TEV protease

Usage and Biology

This part includes the TVMV Protease cleavage site. TVMV cleaves the sequence ETVRFQS. We are using this recognition site to cut our protein in front of its polyG-tag so it has a C-terminal polyG-tag that can be used in a Sortase A reaction. Sequence and Features BBa_K3187020 SequenceAndFeatures The TVMV protease cuts after the final Gln. Cleavage works best if the following amino acid is Gly or Ser, but other amino acids at P1' except proline work as well