Difference between revisions of "Part:BBa K3139001:Design"

 
(→‎References)
 
Line 16: Line 16:
  
 
===References===
 
===References===
 +
[1]Khosa S , Scholz R , Schwarz C , et al. An A/U-rich enhancer region is required for high-level protein secretion through the HlyA Type I secretion system[J]. Applied and Environmental Microbiology, 2017:AEM.01163-17.

Latest revision as of 17:59, 19 October 2019


A/U-rich 5-UTR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The length and A/U content of an A/U-rich 5'-UTR should be various from species, different hosts should have the most suitable one. Our design is to be used in E.coli DH5α. For further application, different mutation should be applied and tested.


Source

E.coli hly operon, mutated from the upstream of the hlyA gene.

References

[1]Khosa S , Scholz R , Schwarz C , et al. An A/U-rich enhancer region is required for high-level protein secretion through the HlyA Type I secretion system[J]. Applied and Environmental Microbiology, 2017:AEM.01163-17.