Difference between revisions of "Part:BBa K3081057"
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dCas9 based replication control on p15A origin plasmid copy number——top strand of initiation site.This part is an improvement of<partinfo>BBa_K3081040</partinfo>, which we add a degradation signal peptide ssrA to the dCas9.  
 
dCas9 based replication control on p15A origin plasmid copy number——top strand of initiation site.This part is an improvement of<partinfo>BBa_K3081040</partinfo>, which we add a degradation signal peptide ssrA to the dCas9.  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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===Functional Parameters===
 
===Functional Parameters===
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Revision as of 17:58, 19 October 2019

CRISPR-based replication interference system for p15A plasmid copy number control, ini(+) site

dCas9 based replication control on p15A origin plasmid copy number——top strand of initiation site.This part is an improvement ofBBa_K3081040, which we add a degradation signal peptide ssrA to the dCas9.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal NheI site found at 2321
    Illegal NheI site found at 5495
    Illegal NheI site found at 5518
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 4600
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961