Difference between revisions of "Part:BBa K2983016"

Revision as of 15:01, 19 October 2019 (view source) Anthor91 (Talk | contribs) (→‎Usage and Biology) ← Older edit		Latest revision as of 17:57, 19 October 2019 (view source) TomZaplana (Talk | contribs)
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	<partinfo>BBa_K2983016 short</partinfo>		<partinfo>BBa_K2983016 short</partinfo>
−	This part is a Loop Type IIS Golden gate adapter that contains a BsaI and a SapI restriction sites.	+	This part is a Loop Type IIS Golden Gate adapter that contains a BsaI and a SapI restriction sites.
	===Usage and Biology===		===Usage and Biology===
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	Blue triangle: SapI fixation site (GCTCTTC)		Blue triangle: SapI fixation site (GCTCTTC)
−	Red triangle : BsaI fixation site (GAGACC)	+	Red triangle: BsaI fixation site (GGTCTC on complementary strand)
−	Blue circle : overhang formed by SapI after cutting (CAG)	+	Blue circle: overhang formed by SapI after cutting (CAG)
−	Red circle: overhang formed by BasI  after cutting (GGAG)	+	Red circle: overhang formed by BsaI after cutting (GGAG)
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	[1] Pollak B, Cerda A, Delmans M, Álamos S, Moyano T, West A, Gutiérrez RA, Patron NJ, Federici F, Haseloff J. Loop assembly: a simple and open system for recursive fabrication of DNA circuits. New Phytol (2019) 222, 628-640.		[1] Pollak B, Cerda A, Delmans M, Álamos S, Moyano T, West A, Gutiérrez RA, Patron NJ, Federici F, Haseloff J. Loop assembly: a simple and open system for recursive fabrication of DNA circuits. New Phytol (2019) 222, 628-640.
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Latest revision as of 17:57, 19 October 2019

Loop Epsilon-A

This part is a Loop Type IIS Golden Gate adapter that contains a BsaI and a SapI restriction sites.

Usage and Biology

The Loop Type IIS cloning sites (triangles in figures 1 and 2) are a combination of BsaI and SapI restriction sites each with different cutting sites that generate well defined overhangs (circles in Figures 1 and 2).

A total of 50 combinations are theoretically possible and this part is one of them: the loop Epsilon-A (Figure 2).

Blue triangle: SapI fixation site (GCTCTTC)

Red triangle: BsaI fixation site (GGTCTC on complementary strand)

Blue circle: overhang formed by SapI after cutting (CAG)

Red circle: overhang formed by BsaI after cutting (GGAG)

This part was used to define the Loop assembly system dedicated to the oleaginous yeast Yarrowia lipolytica (for details visit https://2019.igem.org/Team:Evry_Paris-Saclay/Design)

References

[1] Pollak B, Cerda A, Delmans M, Álamos S, Moyano T, West A, Gutiérrez RA, Patron NJ, Federici F, Haseloff J. Loop assembly: a simple and open system for recursive fabrication of DNA circuits. New Phytol (2019) 222, 628-640.

Sequence and Features