Difference between revisions of "Part:BBa K2951004"
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===Usage and Biology=== | ===Usage and Biology=== | ||
This part is a ssDNA aptamer that targets the nucleocapsid protein of influenza A virus (A/Michigan/297/2017(H1N1)).(sequence from NCBI(CY261696.1) gene:34-1530) | This part is a ssDNA aptamer that targets the nucleocapsid protein of influenza A virus (A/Michigan/297/2017(H1N1)).(sequence from NCBI(CY261696.1) gene:34-1530) | ||
Revision as of 17:30, 19 October 2019
Apt-influenzaA-NP
Usage and Biology
This part is a ssDNA aptamer that targets the nucleocapsid protein of influenza A virus (A/Michigan/297/2017(H1N1)).(sequence from NCBI(CY261696.1) gene:34-1530) The probe can be used for on-site influenza detection.
Characterization
Titer Value
The titer value of this aptamer towards its target protein nucleocaspid is determined by practicing two types of ELISA, according to the coated protein, two important factors are tested:
| Factor | Method |
|---|---|
| Affinity | Non-competitive ELISA by coating target protein |
| Specificity | competitive ELISA by coating un-target protein |
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]