Difference between revisions of "Part:BBa K3311004"
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<partinfo>BBa_K3311004 short</partinfo> | <partinfo>BBa_K3311004 short</partinfo> | ||
| − | + | The “HucR-pHucO-mCherry” is composed of “Anderson Strong-RBS-HucR”(BBa_K3311003) and “pHucO-mCherry”(BBa_K3311002 ). It is a important tool for our part function test. HucR is promoted by a constitutive promoter, while the transcription of mCherry is controlled by pHucO. | |
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K3311004 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3311004 SequenceAndFeatures</partinfo> | ||
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| + | ===Usage and Biology=== | ||
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| + | As for “HucR-pHucO-mCherry” (Figure 2), while uric acid is absent, HucR will bind to pHucO operator, inhibiting the transcription of downstream genes--mCherry. In this situation, the color of the sample will not change. While uric acid is present, it will change the structure of HucR, causing it to leave pHucO, allowing mCherry to transcript, the sample will turn red. | ||
| + | Now, we successfully constructed the plasmid “HucR-pHucO-mCherry”（Figure1-2）. | ||
| + | [[File:HucR-pHucO_1.png|300px|thumb|left|Figure 1 Photos of transformants of pSB1C3-HucR-pHucO-mCherry on LB agar broth.]] | ||
| + | [[File:HucR-pHucO_3.png|300px|thumb|left|Figure 2 Enzyme digestion analysis pSB1C3-HucR-pHucO-mCherry.]] | ||
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Revision as of 17:12, 19 October 2019
HucR-pHucO-mCherry
The “HucR-pHucO-mCherry” is composed of “Anderson Strong-RBS-HucR”(BBa_K3311003) and “pHucO-mCherry”(BBa_K3311002 ). It is a important tool for our part function test. HucR is promoted by a constitutive promoter, while the transcription of mCherry is controlled by pHucO.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
As for “HucR-pHucO-mCherry” (Figure 2), while uric acid is absent, HucR will bind to pHucO operator, inhibiting the transcription of downstream genes--mCherry. In this situation, the color of the sample will not change. While uric acid is present, it will change the structure of HucR, causing it to leave pHucO, allowing mCherry to transcript, the sample will turn red. Now, we successfully constructed the plasmid “HucR-pHucO-mCherry”（Figure1-2）.
