Difference between revisions of "Part:BBa K3081003"
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A fluorescent protein sfGFP is fused to the C-terminal of dCas9 for quantitative characterization. | A fluorescent protein sfGFP is fused to the C-terminal of dCas9 for quantitative characterization. | ||
A degradation tag ssrA is added to the end of sfGFP to alleviate the burden caused by dCas9. | A degradation tag ssrA is added to the end of sfGFP to alleviate the burden caused by dCas9. | ||
| − | The expression of this fusion protein is driven by pBAD promoter, which can be triggered by arabinose. | + | The expression of this fusion protein is driven by pBAD promoter, which can be triggered by arabinose.This part is an improvement of dCas9-linker-sfGFP(<partinfo>BBa_K3081119<partinfo>), in which we add a degradation signal peptide ssrA to dCas9 |
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Revision as of 16:27, 19 October 2019
pBAD-dCas9-linker-sfGFP-ssrA
A fluorescent protein sfGFP is fused to the C-terminal of dCas9 for quantitative characterization. A degradation tag ssrA is added to the end of sfGFP to alleviate the burden caused by dCas9. The expression of this fusion protein is driven by pBAD promoter, which can be triggered by arabinose.This part is an improvement of dCas9-linker-sfGFP(No part name specified with partinfo tag.