Difference between revisions of "Part:BBa K3081002"

 
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<partinfo>BBa_K3081002 short</partinfo>
 
<partinfo>BBa_K3081002 short</partinfo>
  
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This part consists of araC/araBAD promoter and sfGFP fused to the C terminus of dCas9.  
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This part consists of araC/araBAD promoter and sfGFP fused to the C terminus of dCas9. Expression level of dCas9 induced by arabinose is tested by fluorescence of fused dCas9 linker sfGFP.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 16:24, 19 October 2019


pBAD-dCas9-linker-sfGFP

This part consists of araC/araBAD promoter and sfGFP fused to the C terminus of dCas9. Expression level of dCas9 induced by arabinose is tested by fluorescence of fused dCas9 linker sfGFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal NheI site found at 2321
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 4600
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961
    Illegal SapI.rc site found at 5369