Difference between revisions of "Part:BBa K3171171"
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| − | + | The strength of the P1 promoter was measured in the P1-mCherry construct, which was designed by 3A assembly method. A synthetic promoter library was constructed by enhancing the function and inducibility. The screening was done based on fluorescence intensity measured. The best variation of the promoter was determined based on fold change in the fluorescence intensity and low basal level. | |
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K3171171 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3171171 SequenceAndFeatures</partinfo> | ||
Revision as of 16:11, 19 October 2019
Vibrio natriegens native P1 promoter
V. natriegens has been reported to have a remarkable doubling time of 9.8 min. As is the case for the better-studied but slower-growing E. coli, V. natriegens increases its number of ribosomes with the growth rate in order to achieve its extraordinarily high rate of protein synthesis. Multiple mechanisms contribute to this high ribosome synthesis efficiency, including high rRNA gene copy number; strong promoters that contain near-consensus −10, −35, and UP elements; and activation by the transcription factor Fis. In addition, V. natriegens rRNA promoters exhibit the relatively short-lived open-complex characteristic of rRNA promoters in E. coli, potentially contributing to the regulation of these promoters in vivo. The native promoter P1 is constitutive.