Difference between revisions of "Part:BBa K3007000"
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===Usage and Biology===
 
===Usage and Biology===
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==Contribution: WHU-China 2019==
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'''Group:''' WHU-China
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<br>'''Authors:''' Jiongyi He
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<br>'''Summary:''' We confirmed the function of sfGFP(BBa_I746916) and determined the relationship between sfGFP protein concentration and fluorescence intensity.
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<br>'''Documentation:'''
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<br>
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https://static.igem.org/mediawiki/parts/e/e3/SfGFP-WHU.png
  
 
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Revision as of 15:58, 19 October 2019

HucR binding site

It is a special DNA sequence which can interact with HucR protein and repress the expression of downstream gene when uric acid is absent. If uric acid presents, the formation of HucR-hucO complex can be reduced and restore downstream gene expression. In our design, we used the HucR-hucO system as a detector for uric acid.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]