Difference between revisions of "Part:BBa K2969000"

 
Line 1: Line 1:
 +
__NOTOC__
 +
<partinfo>BBa_K2969000 short</partinfo>
  
 +
TEV protease is a highly specific cysteine protease from Tobacco Etch Virus that recognizes the amino-acid sequence Glu-Asn-Leu-Tyr-Phe-Gln-(Gly/Ser) and cleaves between the Gln and Gly/Ser residues. It is often used for the removal of affinity purification tags such as maltose-binding protein (MBP) or poly-histidine from fusion proteins. In our project we insert the recognition sequence into the transcription factors so that the expression of protease can inhibit the action of transcription factors.
 +
 +
<!-- Add more about the biology of this part here
 +
===Usage and Biology===
 +
 +
<!-- -->
 +
<span class='h3bb'>Sequence and Features</span>
 +
<partinfo>BBa_K2969000 SequenceAndFeatures</partinfo>
 +
 +
 +
<!-- Uncomment this to enable Functional Parameter display
 +
===Functional Parameters===
 +
<partinfo>BBa_K2969000 parameters</partinfo>
 +
<!-- -->

Revision as of 15:23, 19 October 2019

TEV protease

TEV protease is a highly specific cysteine protease from Tobacco Etch Virus that recognizes the amino-acid sequence Glu-Asn-Leu-Tyr-Phe-Gln-(Gly/Ser) and cleaves between the Gln and Gly/Ser residues. It is often used for the removal of affinity purification tags such as maltose-binding protein (MBP) or poly-histidine from fusion proteins. In our project we insert the recognition sequence into the transcription factors so that the expression of protease can inhibit the action of transcription factors.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 319
    Illegal SapI.rc site found at 667