Difference between revisions of "Part:BBa J70025:Design"
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Alignment of the promoter with the coding region, preservation of the RBS sites. | Alignment of the promoter with the coding region, preservation of the RBS sites. | ||
| − | + | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGggccagtctacatgtactc,J00025-F | |
| − | + | GTTTCTTCCTGCAGCGGCCGCTACTAGTAttcctccattcaaaaacccaa,J00025-R | |
===Source=== | ===Source=== | ||
Revision as of 18:38, 20 October 2008
Promoter for tetM gene, from pBOT1 plasmid, pAMbeta1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Alignment of the promoter with the coding region, preservation of the RBS sites.
GTTTCTTCGAATTCGCGGCCGCTTCTAGAGggccagtctacatgtactc,J00025-F GTTTCTTCCTGCAGCGGCCGCTACTAGTAttcctccattcaaaaacccaa,J00025-R
Source
pBOT1 plasmid PCR. pBOT1 from J. Renaudin. Original sequence is part of pAMbeta1 tetracycline resistance plasmid.
References
Su YA, He P, Clewell DB. Characterization of the tet(M) determinant of Tn916: evidence for regulation by transcription attenuation. Antimicrob Agents Chemother. 1992 Apr;36(4):769-78. PMID: 1323953