Revision as of 15:20, 19 October 2019

AOX1-Kozak-FLO10-pelA-His tag-AOX1 Terminator

The SUC2 gene of S. cerevisiae, encoding invertase was designed to codon preference of P. pastoris excluding the sequence of the N-terminal pre-pro-sequence signal peptide and His-tag in the C terminus. pelA can catalyze pectin.

Characterization

This is a four section for degrade and transfer lignin part.

Figure1. T--HUST--China--2019-FLO10pelA

DNA Gel Electrophoretic

After we link SUC2 and pelA successfully, we run the PCR with an intention to confirm the expression of pelA. As the figure shows, we get the genetic stripe about xxx bp which means the PCR is successful.

Figure1. This figure shows the most possible kozak consensus sequence.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1787
Illegal BamHI site found at 937
Illegal BamHI site found at 948
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 13: / Line 13: @@
 [[File:Kozak_sequence.png ‎|400px|thumb|center|Figure1. This figure shows the most possible kozak consensus sequence.]]
-<h1>'''SDS-PAGE'''</h1>
-We run the SDS-PAGE to check whether SUC2 help the enzyme transfer to the extracellular. As the figure shows, we get the protein type about xxx bp which means the SDS-PAGE is successful.
-[[File:Kozak_sequence.png ‎|400px|thumb|center|Figure1. This figure shows the most possible kozak consensus sequence.]]
-<h1>'''Enzyme Activity'''</h1>
-We use DNS to detect the enzyme activity. As the figure shows, the solution turns xxx, which confirm the enzyme activity.
-[[File:Kozak_sequence.png ‎|400px|thumb|center|Figure1. This figure shows the most possible kozak consensus sequence.]]

Difference between revisions of "Part:BBa K3196016"

Revision as of 15:20, 19 October 2019

Characterization

DNA Gel Electrophoretic