Difference between revisions of "Part:BBa J70024:Design"

 
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===References===
 
===References===
 +
Sanchez-Pescador,R., Brown,J.T., Roberts,M. and Urdea,M.S.
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The nucleotide sequence of the tetracycline resistance determinant
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tetM from Ureaplasma urealyticum
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Nucleic Acids Res. 16 (3), 1216-1217 (1988)
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PMID: 3344217

Revision as of 18:07, 20 October 2008

TetM gene, recoded compromise E. coli & Mesoplasma florum


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Elimination of restriction sites, CGG codons, UGA codons, rare codons in both species.


Source

Synthesized from the Ureaplasma urealyticum tetM gene sequence, with recoding.

References

Sanchez-Pescador,R., Brown,J.T., Roberts,M. and Urdea,M.S. The nucleotide sequence of the tetracycline resistance determinant tetM from Ureaplasma urealyticum Nucleic Acids Res. 16 (3), 1216-1217 (1988) PMID: 3344217