Difference between revisions of "Part:BBa K3196021"
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<h1>'''Characterization'''</h1> | <h1>'''Characterization'''</h1> | ||
This is a four section for degrade and transfer lignin part. | This is a four section for degrade and transfer lignin part. | ||
− | [[File: | + | [[File:T--HUST--China--2019-FLO10VP.jpg |400px|thumb|center|Figure1. T--HUST--China--2019-FLO10VP]] |
<h1>'''DNA Gel Electrophoretic'''</h1> | <h1>'''DNA Gel Electrophoretic'''</h1> | ||
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[[File:Kozak_sequence.png |400px|thumb|center|Figure1. This figure shows the most possible kozak consensus sequence.]] | [[File:Kozak_sequence.png |400px|thumb|center|Figure1. This figure shows the most possible kozak consensus sequence.]] | ||
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Revision as of 15:10, 19 October 2019
AOX1-Kozak-FLO10-VP-His tag-AOX1 Terminator
FLO10 is a Lectin-like protein with similarity to Flo1 p, thought to be expressed and involved in flocculation. VP can catalyze pectin.
Characterization
This is a four section for degrade and transfer lignin part.
DNA Gel Electrophoretic
After we link FLO10 and VP successfully, we run the PCR with an intention to confirm the expression of VP. As the figure shows, we get the genetic stripe about xxx bp which means the PCR is successful.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 937
Illegal BamHI site found at 948 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]