Difference between revisions of "Part:BBa K3081116"
| Line 3: | Line 3: | ||
<partinfo>BBa_K3081116 short</partinfo> | <partinfo>BBa_K3081116 short</partinfo> | ||
| − | + | The 20 bp sgRNA targets to the linker sequence of two DnaA binding boxes (M and R2) on E.coli genome replication initiation region, OriC. Not considered to be DnaA binding box,blocking this site has no inhibition on cell growth. For more detailed information, see <partinfo>BBa_K3081058</partinfo> | |
| + | |||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 15:03, 19 October 2019
a kind of 20-bp sgRNA that is designed to be complementary to OriC, the genome replication origin
The 20 bp sgRNA targets to the linker sequence of two DnaA binding boxes (M and R2) on E.coli genome replication initiation region, OriC. Not considered to be DnaA binding box,blocking this site has no inhibition on cell growth. For more detailed information, see BBa_K3081058
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]